Three new and eleven known unusual C25 steroids: activated production of silent metabolites in a marine-derived fungus by chemical mutagenesis strategy using diethyl sulphate.
作者： Ming-Wen Xia , Cheng-Bin Cui , Chang-Wei Li , Chang-Jing Wu
DOI: 10.3390/MD12031545
关键词:
摘要: Three new (1–3) and 11 known (4–14) C25 steroids with an unusual bicyclo[4.4.1]A/B ring system were isolated by tracing newly produced metabolites in the EtOAc extract of antitumor mutant AD-1-2 obtained diethyl sulphate (DES) mutagenesis a marine-derived Penicillium purpurogenum G59. HPLC-PDAD-UV HPLC-ESI-MS analyses indicated that G59 strain did not produce these production 1–14 was caused activation silent original DES mutagenesis. The structures compounds, named antineocyclocitrinols A (1) B (2) 23-O-methylantineocyclocitrinol (3), including their absolute configurations determined various spectroscopic methods, especially NMR Mo2-induced CD analyses. Compounds 1–3 provide first examples Z-configuration 20,22-double bond. All weakly inhibited several human cancer cell lines to varying extents. These results provided additional for successful application chemical strategy using discover compounds activating fungal isolates supported also effectiveness usefulness this strategy.
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