Confocal raman microspectroscopy and imaging study of theraphthal in living cancer cells.

摘要: Abstract Binary systems combining a transition metal complex and ascorbate have been proposed recently for catalytic therapy of malignant tumors. The killing effect on tumor cells is achieved by production free radicals in the course accelerated oxidation dioxygen presence complexes. Further progress development binary (BCSs) requires special method their investigation tissues, because neither component BCSs fluoresces. Here resonance Raman confocal spectral imaging (RR CSI) technique was introduced as unique approach to monitor quantitatively complexes within living cells. Intracellular accumulation, localization, retention theraphthal (TP), catalyst advanced TP/ascorbate BCS, were investigated A549 with RR CSI technique. cellular analysis complemented detailed study molecular interactions TP solution environmental factors affecting spectrum TP. does not penetrate into membranes, it binds very weakly DNA RNA, but readily forms proteins. Binding Ca 2+ cations decreasing pH below 6 induce aggregation By analyzing spectra recorded from every point TP-treated cell, three states agent discriminated, namely, monomeric polar environment, bound proteins, aggregated Their cytoplasmic nuclear distributions mapped at different stages uptake efflux. introducing organelle-selective fluorescent probes drug-treated measuring intracellular localization both probe drug, compartmentation revealed. Cell growth suppression system measured, probable organelle targets radical damage characterized.