Conservation of Cdc14 phosphatase specificity in plant fungal pathogens: implications for antifungal development.

摘要: Cdc14 protein phosphatases play an important role in plant infection by several fungal pathogens. This and other properties of enzymes make them intriguing target for development new antifungal crop treatments. Active site architecture substrate specificity from the model fungus Saccharomyces cerevisiae (ScCdc14) are well-defined unique among characterized phosphatases. appears absent some plants. However, extent conservation sequence, structure, pathogens is unknown. We addressed this performing a comprehensive phylogenetic analysis family comparing active structure sampling pathogen homologs. show that was lost common ancestor angiosperm plants but ubiquitous ascomycete basidiomycete fungi. The ScCdc14 invariant homologs eight diverse species dikarya, suggesting it conserved across lineage. A synthetic mimetic inhibited with similar low µM Ki values, had little effect on related Our results justify future exploration as broad spectrum protection.