Temporal Dynamics of Bacterial Communities in Soil and Leachate Water After Swine Manure Application

摘要: Application of swine manure to agricultural land allows recycling plant nutrients, but excess nitrate, phosphorus and fecal bacteria impact surface drainage water quality. While agronomic quality impacts are well studied, little is known about the slurry on soil microbial communities. We applied intact columns collected from plots maintained under chisel plow or no-till with corn soybean rotation. Targeted 16S-rRNA gene sequencing was used characterize identify shifts in bacterial communities over 108 days after application. In addition, six simulated rainfalls were during this time. Drainage sampled, DNA extracted sequenced. Unique sequences (OTU) associated 12 orders responsible for majority OTUs stimulated by Proteobacteria most prevalent, followed Bacteroidetes, Firmicutes, Actinobacteria, Spirochaetes. decreased day 59, relative abundances genes Rhizobiales Actinomycetales increased. Bacterial which application had varied responses waters course experiment. also identified a "manure-specific core" five genera who comprised 13% community not significantly abundant non-manured control soils. Of these genera, Clostridium sensu stricto only genus did return pre-manure abundance 108. Our results show that enrichment amendment could result displacement native manure-borne process growth using manure-derived available nutrients.