Perinuclear cytoskeleton of acrosome-less spermatids in the blind sterile mutant mouse.

摘要: The perinuclear cytoskeleton of mammalian spermatids is thought to play a major role in nucleus-acrosome association and shape changes the head during spermiogenesis. To test these hypotheses acrosome-less blind-sterile mutant mice were investigated for development subacrosomal layer. Immunogold procedures used detection actin calmodulin. In addition various other abnormalities many round elongating developed layer with an calmodulin distribution similar that observed normal spermatids. However, apical part nucleus was truncated and/or folded. expected elongation shaping only occurred its caudal associated hypertrophied somewhat ectopic manchette. These those previously experimental models indicated may form independently acrosome. It suggested filamentous transitory scaffolding which might be involved assemblage proteins cytoskeleton. by itself, this not sufficient ensure nucleus. Acrosome-nucleus interactions mediated seem necessary cranial spermatid head. manchette appears nuclear shaping.