NF-κB Signaling Regulates Functional Expression of the MHC Class I-Related Neonatal Fc Receptor for IgG via Intronic Binding Sequences

摘要: The neonatal Fc receptor for IgG (FcRn) functions to transport maternal a fetus or newborn and protect from degradation. Although FcRn is expressed in variety of tissues cell types, the extent which expression regulated by immunological inflammatory events remains unknown. Stimulation intestinal epithelial lines, macrophage-like THP-1, freshly isolated human monocytes with cytokine TNF-α rapidly up-regulated gene expression. In addition, TLR ligands LPS CpG oligodeoxynucleotide enhanced level THP-1 monocytes. Treatment TNF-stimulated cells NF-κB-specific inhibitor overexpression dominant negative mutant inhibitory NF-κB (IκBα; S32A/S36A) resulted down-regulation By using chromatin immunoprecipitation we identified three binding sequences within introns 2 4 gene. An EMSA confirmed p50/p50 and/or p65/p50 complex (s) bound intron 2- 4-derived oligonucleotides containing putative sequences, respectively. intronic combination promoter alone luciferase reporter response stimulation p65 p50. DNA looping interactions potentially occurred after between as shown chromosome conformation capture assay. Finally, stimulations across an Caco-2 monolayer. Together, these data provide first evidence that signaling via regulates function.