Single-Tube 10-Fluorochrome Analysis for Efficient Flow Cytometric Evaluation of Minimal Residual Disease in Plasma Cell Myeloma

摘要: Objectives: Widespread adoption of recent recommendations for minimal residual disease (MRD) detection in myeloma has partly been impeded by a paucity studies detailing multiparameter flow cytometry (MPF) assay validation. In response, we have validated novel and efficient single-tube 10-color MRD that incorporates the recently recommended plasma cell markers. Methods: Aspirate samples from 53 patients with disorder were analyzed using method. The limit detection, precision measurement, linearity measurement our new determined serial dilution experiments. stability antibody cocktail viability/specificity stained evaluated time course measurements. Results: There was high degree quantitative agreement between method an established eight-color Four positive detected below or at assay, confirming its sensitivity. two cases, subsequent revision International Myeloma Working Group Uniform Response Criteria necessary. Conclusion: Adoption would enable clinical laboratories to satisfy current without significantly increasing demands on workflow practices.