Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV

作者: Nattaphon Busayarat , Saengchan Senapin , Moltira Tonganunt , Kornsunee Phiwsaiya , Watcharachai Meemetta

DOI: 10.1016/J.FSI.2011.03.007

关键词:

摘要: Laminin receptor (Lamr) in shrimp was previously proposed to be a potential protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since Lamr bound the VP1 capsid of TSV, we were interested know whether capsid/envelope proteins from other viruses would also bind Lamr. Thus, encoding genes 5 additional examined. These Penaeus stylirostris densovirus (PstDNV), white spot (WSSV), infectious myonecrosis (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head (YHV). Protein interaction analysis using assay revealed that specifically interacted with RNA IMNV YHV but not MrNV DNA PstDNV WSSV. In vitro pull-down confirmed between gp116 envelope protein, injection recombinant (rLamr) produced cells protected against laboratory challenge tests.

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