The α-Chemokine, Stromal Cell-derived Factor-1α, Binds to the Transmembrane G-protein-coupled CXCR-4 Receptor and Activates Multiple Signal Transduction Pathways
作者： Ramesh K. Ganju , Stephanie A. Brubaker , Joshua Meyer , Parmesh Dutt , Yangming Yang
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摘要: The α-chemokine stromal cell-derived factor (SDF)-1α binds to the seven transmembrane G-protein-coupled CXCR-4 receptor and acts modulate cell migration proliferation. signaling pathways that mediate effects of SDF-1α are not well characterized. We studied events following binding in a model murine pre-B line transfected with human CXCR-4. There was enhanced tyrosine phosphorylation association components focal adhesion complexes such as related kinase, paxillin, Crk. also observed activation phosphatidylinositol 3-kinase. Wortmannin, selective inhibitor 3-kinase, partially inhibited SDF-1α-induced paxillin. treatment selectively activated p44/42 mitogen-activated protein kinase (Erk 1 Erk 2) its upstream but p38 c-Jun amino-terminal or mitogen kinase. increased NF-κB activity nuclear extracts from transfectants. Taken together, these studies revealed activates distinct may growth, migration, transcriptional activation.
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