Inhibition of ATP release from Erythrocytes: A role for EPACs and PKC
作者： SHAQURIA P. ADDERLEY , MEERA SRIDHARAN , ELIZABETH A. BOWLES , ALAN H. STEPHENSON , RANDY S. SPRAGUE
DOI: 10.1111/J.1549-8719.2010.00073.X
关键词:
摘要: Please cite this paper as: Adderley, Sridharan, Bowles, Stephenson, Sprague and Ellsworth (2011). Inhibition of ATP Release from Erythrocytes: A Role for EPACs PKC. Microcirculation18(2), 128–135. Abstract Objective: Here we demonstrate that, in human erythrocytes, increases cAMP that are not localized to a specific receptor-mediated signaling pathway release can activate effector proteins resulting inhibition release. Specifically sought establish exchange activated by (EPACs) inhibit via activation protein kinase C (PKC). Methods: stimulated iloprost (ILO), or isoproterenol (ISO), was determined the absence presence selective phosphodiesterase inhibitors and/or EPAC activator, 8CPT2OMecAMP (8CPT). To determine whether PKC, erythrocytes were incubated with phorbol 12-myristate 13-acetate (PMA) prior either forskolin ILO PKC inhibitor, calphostin (CALC). Results: Selective PDEs one inhibited response other cAMP-dependent pathway. 8CPT PMA both ILO- ISO-induced rescued CALC. Conclusion: These results support hypothesis which suggest novel role erythrocytes.
sci-hub.se 参考文章(53)
M M Hosey, M Tao, Phosphorylation of rabbit and human erythrocyte membranes by soluble adenosine 3':5'-monophosphate-dependent and -independent protein kinases. Journal of Biological Chemistry. ,vol. 252, pp. 102- 109 ,(1977) , 10.1016/S0021-9258(17)32804-1
Martina Schmidt, Sandrine Evellin, Paschal A. Oude Weernink, Frank vom Dorp, Holger Rehmann, Jon W. Lomasney, Karl H. Jakobs, A new phospholipase-C-calcium signalling pathway mediated by cyclic AMP and a Rap GTPase. Nature Cell Biology. ,vol. 3, pp. 1020- 1024 ,(2001) , 10.1038/NCB1101-1020
Joseph A. Beavo, Laurence L. Brunton, Cyclic nucleotide research — still expanding after half a century Nature Reviews Molecular Cell Biology. ,vol. 3, pp. 710- 717 ,(2002) , 10.1038/NRM911
Johannes L. Bos, Epac: a new cAMP target and new avenues in cAMP research Nature Reviews Molecular Cell Biology. ,vol. 4, pp. 733- 738 ,(2003) , 10.1038/NRM1197
George S. Baillie, Compartmentalized signalling: spatial regulation of cAMP by the action of compartmentalized phosphodiesterases. FEBS Journal. ,vol. 276, pp. 1790- 1799 ,(2009) , 10.1111/J.1742-4658.2009.06926.X
A Waseem, H C Palfrey, Protein kinase C in the human erythrocyte. Translocation to the plasma membrane and phosphorylation of bands 4.1 and 4.9 and other membrane proteins. Journal of Biological Chemistry. ,vol. 260, pp. 16021- 16029 ,(1985) , 10.1016/S0021-9258(17)36360-3
Bernard L. Strehler, Bioluminescence Assay: Principles and Practice Methods of Biochemical Analysis. ,vol. 16, pp. 99- 181 ,(2006) , 10.1002/9780470110348.CH2
R. S. Sprague, M. L. Ellsworth, A. H. Stephenson, A. J. Lonigro, ATP: the red blood cell link to NO and local control of the pulmonary circulation. American Journal of Physiology-heart and Circulatory Physiology. ,vol. 271, ,(1996) , 10.1152/AJPHEART.1996.271.6.H2717
Johan de Rooij, Fried J. T. Zwartkruis, Mark H. G. Verheijen, Robbert H. Cool, Sebastian M. B. Nijman, Alfred Wittinghofer, Johannes L. Bos, Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP Nature. ,vol. 396, pp. 474- 477 ,(1998) , 10.1038/24884
Mélanie Métrich, Magali Berthouze, Eric Morel, Bertrand Crozatier, Ana Maria Gomez, Frank Lezoualc’h, Role of the cAMP-binding protein Epac in cardiovascular physiology and pathophysiology Pflügers Archiv - European Journal of Physiology. ,vol. 459, pp. 535- 546 ,(2010) , 10.1007/S00424-009-0747-Y