Fabrication of UV-crosslinked chitosan scaffolds with conjugation of RGD peptides for bone tissue engineering

摘要: In this study, a simple process was established to fabricate RGD-conjugated and crosslinked chitosan scaffolds for bone tissue engineering. Two types of derivatives were synthesized: one containing photoreactive azido groups the other tethered with RGD. Their mixture fabricated into by freeze-drying UV crosslinking. The pore size mechanical property increased 1.36 2.41 folds, respectively, post-crosslinking process. Incorporation RGD enhanced culture rat primary osteoblasts. After 10 days culture, cell number in RGD-contained 50% compared control. Furthermore, calcium deposition osteoblasts RGD-incorporated almost doubled control, thus greatly scaffolds. Our technique is proved useful preparing engineering biomedical applications.