Microdevice Platform for Continuous Measurement of Contractility, Beating Rate, and Beating Rhythm of Human-Induced Pluripotent Stem Cell-Cardiomyocytes inside a Controlled Incubator Environment
作者： Li Wang , Wenkun Dou , Manpreet Malhi , Min Zhu , Haijiao Liu
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摘要: The heart completes a complex set of tasks, including the initiation or propagation an electrical signal with regularity (proper rate and rhythm) generating sufficient force contraction (contractility). Probing mechanisms diseases quantifying drug efficacies demand platform that is capable continuous operation inside cell incubator for long-term measurement cardiomyocyte (CM) monolayers. Here, we report microdevice array performing continuous, (14 days) contractility, beating rate, rhythm in monolayer human-induced pluripotent stem cell-CMs (hiPSC-CMs). device consists deformable membrane embedded carbon nanotube (CNT)-based strain sensors. Contraction hiPSC-CMs seeded on induces resistance change CNT sensor. Continuously reading sensor signals revealed started to beat from day 2 plateaued 5. Average contractile stres...
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