High Precision, Electrochemical Detection of Reversible Binding of Recombinant Proteins on Wide Bandgap GaN Electrodes Functionalized with Biomembrane Models

摘要: We report a novel hybrid charge sensor realized by the deposition of phospholipid monolayers on highly doped n-GaN electrodes. To detect binding recombinant proteins with histidine-tags, lipid vesicles containing chelator lipids were deposited GaN electrodes pre-coated octadecyltrimethoxysilane monolayers. Owing to its optical transparency, allows confirmation fluidity supported membranes fluorescence recovery after photo-bleaching (FRAP). The electrolyte-(organic) insulator-semiconductor (EIS) setup enables one transduce variations in surface density ΔQ into change interface capacitance ΔC p and, thus, flat-band potential ΔU FB. obtained results demonstrate that membrane-based can reach high sensitivity reversible changes formation complexes, docking eGFP histidine tags, and cancellation EDTA. achievable resolution ≥ 0.1 μC/cm2 is better than for membrane-functionalized p-GaAs, 0.9 μC/cm2, ITO coated polymer monolayer, 2.2 μC/cm2. Moreover, we examined application optically active InGaN/GaN quantum dot structures, detection from photoluminescence signals measured at room temperature.