Characterization of rumen bacterial diversity and fermentation parameters in concentrate fed cattle with and without forage.

摘要: Aims:  To determine the effects of removal forage in high-concentrate diets on rumen fermentation conditions and bacterial populations using culture-independent methods. Methods Results:  Detectable bacteria parameters were measured solid liquid fractions digesta from cattle fed two dietary treatments, high concentrate (HC) without (HCNF). Comparison showed that duration time spent below pH 5·2 osmolality higher HCNF treatment. Simpson’s index 16S PCR-DGGE images a greater diversity dominant species Real-time qPCR Fibrobacter succinogenes (P = 0·01) lower than HC diets. Ruminococcus spp., F. succinogenes Selenomonas ruminantium at (P ≤ 0·05) concentrations vs fraction regardless diet. Conclusions:  The detectable community structure is highly diverse. Reducing diet complexity by removing increased despite associated reduction ruminal being less conducive for fibrolytic populations. Quantitative PCR resulted decline density some, but not all examined. Significance Impact Study:  Molecular techniques such as DGGE provide an understanding impacts changes nature populations, conclusions derived these may match those previously traditional laboratory culturing techniques.