Properties of syringomycin, a wide spectrum antibiotic and phytotoxin produced by Pseudomonas syringae, and its role in the bacterial canker disease of peach trees

摘要: Abstract Methods were devised for the large-scale production of syringomycin using stainless steel trays which poured with agar medium, and then covered cellophane. The bacteria grown on surface Syringomycin was extracted from cellophane purified by ion exchange column chromatography partition chromatography; it a single, low molecular weight, ninhydrin-reactive compound. Nine spots detected in acid hydrolysate two-dimensional chromatography. Eight corresponded RF color to amino acids tentatively identified as aspartic acid, d -glutamic serine, glycine, α-alanine, valine, -phenylalanine -lysine. Investigation some properties showed that its antibiotic activity labile alkaline solutions, especially above pH 8. is soluble water lower alcohols, insoluble ether chloroform. Its isoelectric point about 7. has wide spectrum activity. A species each six genera bacteria, four different fungal species, unicellular algal Daphnia all inhibited levels syringomycin. growth Geotrichum candidum, most sensitive organisms surveyed, completely 24 μg per ml. produced phytotoxic symptoms leaves peach shoots when stems immersed h solution containing 600 became brown necrotic after being one week 6 Inoculations Pseudomonas syringae bacterium very similar Inactivation antifungal mild manipulation also decreased phytotoxicity proportionately. An indistinguishable isolated diseased host tissues. It concluded probably major phytotoxin bacterial canker disease trees.