Molecular imprinted polymeric porous layers in open tubular capillaries for chiral separations

摘要: A new method has been developed for the preparation of molecular imprinted polymers as porous layers in open tubular (MIP-PLOT) capillary column formats use chiral separations by liquid chromatography. The synthesis was based on ‘in-capillary’ ultraviolet (UV) initiated polymerization using light emitting diodes (LEDs) conjunction with continuous delivery pre-polymerization reagents into zone an automated device. relationships between exposure times, UV-light intensity and polymer layer thickness have determined, well effects reagent rate multiple LED exposures various compositions mixtures. surface morphology investigated scanning electron microscopy (SEM). non-steroidal anti-inflammatory drug S-ketoprofen used template MIP PLOT coatings. separation performance ketoprofen racemate In contrast to alternative methods, which require expensive selectors, described stationary phases non-chiral precursors create enantioselective nano-cavities through self-assembly processes. fabrication methods provide a avenue tailor-make MIP-PLOT columns compounds present complex or racemic analyte mixtures chemical biological origin.