Studies On Immobilized Enzymes For Biosensor Applications

作者: M. Devaraja Gouda

DOI:

关键词:

摘要: In the context of usage immobilized enzymes for sugar biosensor applications, present investigations attempt to a better understanding various aspects enzyme applications. The work has involved construction an based glucose and sucrose in food fermentation analysis, study effect various parameters on performance also basic biochemical aspects single multienzyme systems with respect temperature, pH, denaturants as well operational stability. Aspects considered include: Construction optimization biosensor, Estimation presence glucose, Stability systems, Thermal stability studies using Mechanism thermal inactivation GOD, Reversible denaturation multienzyme systems,

参考文章(13)
Gabriele Wagner, George G. Guilbault, Food biosensor analysis. M. Dekker. ,(1994)
Charles S. Craik, Jeffrey L. Cleland, Protein engineering : principles and practice Wiley-Liss. ,(1996)
M. N. Gupta, Thermostability of enzymes Springer Verlag , Narosa Publishing House. ,(1993)
MD Gouda, MS Thakur, NG Karanth, None, Optimization of the multienzyme system for sucrose biosensor by response surface methodology. World Journal of Microbiology & Biotechnology. ,vol. 17, pp. 595- 600 ,(2001) , 10.1023/A:1012473522593
Jonathan M. Cooper, Anthony E.G. Cass, Biosensors : a practical approach Oxford University Press. ,(2004)
Roy Lester Whistler, Melville Lawrence Wolfrom, Derek H. Shaw, James N. BeMiller, Methods in Carbohydrate Chemistry ,(1965)
Ikuo Satoh, Isao Karube, Shuichi Suzuki, Enzyme electrode for sucrose Biotechnology and Bioengineering. ,vol. 18, pp. 269- 272 ,(1976) , 10.1002/BIT.260180211
Isao Karube, Anthony PF Turner, George S Wilson, None, Biosensors: fundamentals and applications Analytica Chimica Acta. ,vol. 201, pp. 363- 364 ,(1987) , 10.1016/S0003-2670(00)85361-1