Differential Ca2+ Influx, KCa Channel Activity, and Ca2+ Clearance Distinguish Th1 and Th2 Lymphocytes
作者： Christopher M. Fanger , Amber L. Neben , Michael D. Cahalan
DOI: 10.4049/JIMMUNOL.164.3.1153
关键词:
摘要: In Th1 and Th2 lymphocytes, activation begins with identical stimuli but results in the production of different cytokines. The expression some cytokine genes is differentially induced according to amplitude pattern Ca2+ signaling. Using fura- 2 imaging murine clones, we observed that rise elicited following store depletion thapsigargin significantly lower cells than cells. Maximal influx rates whole-cell currents showed both express indistinguishable Ca2+-release-activated channels. Therefore, investigated other mechanisms controlling concentration intracellular Ca2+, including K+ channels clearance from cytosol. Whole-cell recording demonstrated there no distinction amplitudes voltage-gated two cell types. Ca2+-activated (KCa) currents, however, were smaller Pharmacological equalization types reduced did not completely eliminate difference between responses, suggesting divergence an additional regulatory mechanism. analyzed cytosol found extrude more quickly combination a faster mechanism accounts for response compared
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