Chemical mutagenesis at the thymidine kinase locus in L5178Y mouse lymphoma cells: Results for 31 coded compounds in the national toxicology program

摘要: Experimental data from the testing of 31 chemicals for mutagenicity at TK locus in L5178Y mouse lymphoma cells are presented and evaluated. If mutagenic activity was not obtained chemical added to suspension cultures 4 hr, then repeated presence hepatic S9 mix prepared Aroclor 1254-induced male Fischer 344 rats. Multiple trials were performed each chemical, treatments analyzed induction small large mutant colony populations. Twelve detected as mutagenic, one (ascorbic acid) questionable, 18 evaluated mutagenic. These results used evaluations by Tennant et al. [Science 236:933–941, 1987] a critical comparison four vitro genotoxicity assays with rodent carcinogenicity results. The assay general agreement studies. Discordant respect (four false negatives six positives) discussed standpoint how predictive performance mutation might be improved.