Enhanced bioconversion of colchicine to regiospecific 3-demethylated colchicine (3-DMC) by whole cell immobilization of recombinant E. coli harboring P450 BM-3 gene
作者： Kashyap Kumar Dubey , Arshad Jawed , Shafiul Haque
DOI: 10.1016/J.PROCBIO.2013.06.001
关键词:
摘要: Abstract Biotransformation of colchicine into regiospecific 3-demethylated (3-DMC) which is pharmacologically active and a potent anti-cancer drug, mediated by immobilization recombinant microbial monooxygenases novel promising strategy for its production. In the present study, Escherichia coli expressing P450 BM-3 was immobilized in calcium-alginate beads ability to catalyze 3-DMC investigated. Characteristics system showed that optimum conditions activity cells were not affected due immobilization. The pH temperature both free found be 7.5 37.5 °C, respectively. Experimental variables under consideration such as Ca2+ concentration, alginate enzyme concentration optimized using response surface methodology. exhibited markedly improved thermal stability compared cells. yield with an average 69%, 82, 73 52% across three independent batches succession against bioconversion cells, indicated operational reusability batch processes. Additionally, packed bed reactor has been proposed biocatalytic other biochemicals.
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