Characterization of the enzyme activity in barley anthocyanin pathway and reaction of these pigments to electromagnetic irradiation

摘要: The enzymatic activities were indirectly investigated in the grain anthocyanin synthesis of blue barley by HPLC data. These results then evaluated together with absorbed light energy levels pigments. In addition, two photochemical experiments, change aleurone pigmentation and water activation was studied at dormant grains, using focused sunlight heat radiation. middle stage (at day after flowering, DAF 26), there a significant cooling period weather. Differences dynamics found before period. production (DAF 26–33) more intense compared to beginning (17–22). degradation detectable during (22–26) than what observed end seed maturation (33–39). most efficient binders glucosides (delphinidin- cyanidin-3-glu.) produced degraded dynamically their complex forms (malonylglucosides, rutinoside). Furthermore, among pigments, cyanidins are able provide greater absorption. dynamism compounds indicate that individual enzymes not multienzyme operate last phase pathway, operation, they can absorption level aleurone. irradiation grains (~ 400  ~ 2500 nm, 2–3 min), anthocyanins facilitated vitrified activation. During (~ 8000 nm, 1 min), laser scattering associated content decreased intensively within white, indicating IR surplus for Observation suggests pigments contribute transfer direction water, so may have role enhancing dissipation.