Relaxation of the folding of globulin around heme of hemoglobin of Homo sapiens by the food-grade additive molecule chlorophyllin
作者： Md. Selim , Arpita Sengupta Sadhu , Kalyan K. Mukherjea
DOI: 10.1007/S00706-010-0339-8
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摘要: Binding of chlorophyllin (Chln), a food-grade additive molecule with hemoglobin (Hb), has been studied by photophysical and photochemical methods view to unravel the biochemical transport pathway it. The binding affinity constant sites between Chln Hb are determined found be 3.3 × 105 M−1 15 (on tryptophan basis), respectively. Fluorimetric quenching experiments entail that is bound in vicinity residue Hb. Circular dichroism studies suggest induces change α-helical content Chlorophyllin hemoglobin, which confirmed from spectrofluorimetric studies, when fluorescence occurs because chlorophyllin-induced exposure hydrophillic zone. cyclic voltammetric indicate redox reaction FeII inhibited shielding it molecule.
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sci-hub.se 参考文章(33)
T H Huang, A G Redfield, NMR study of relative oxygen binding to the alpha and beta subunits of human adult hemoglobin. Journal of Biological Chemistry. ,vol. 251, pp. 7114- 7119 ,(1976) , 10.1016/S0021-9258(17)32949-6
Eric Ponder, Hemolysis and related phenomena ,(1948)
Giuseppe Geraci, Lawrence J. Parkhurst, [17] Circular dichroism spectra of hemoglobins Methods in Enzymology. ,vol. 76, pp. 262- 275 ,(1981) , 10.1016/0076-6879(81)76127-5
Tomoe Negishi, Sakae Arimoto, Chiharu Nishizaki, Hikoya Hayatsu, Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5H-pyrido[4,3-b indole (Trp-P-2) Carcinogenesis. ,vol. 10, pp. 145- 149 ,(1989) , 10.1093/CARCIN/10.1.145
E.A. Kapp, S. Daya, C.G. Whiteley, Protein-ligand interactions: Interaction of nitrosamines with nicotinic acetylcholine receptor Biochemical and Biophysical Research Communications. ,vol. 167, pp. 1383- 1392 ,(1990) , 10.1016/0006-291X(90)90676-E
Kwang-Kyun Park, Jae-Hee Park, Youn-Joo Jung, Won-Yoon Chung, Inhibitory effects of chlorophyllin, hemin and tetrakis(4-benzoic acid)porphyrin on oxidative DNA damage and mouse skin inflammation induced by 12-O-tetradecanoylphorbol-13-acetate as a possible anti-tumor promoting mechanism. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 542, pp. 89- 97 ,(2003) , 10.1016/J.MRGENTOX.2003.09.001
C. E. Castro, Ruth S. Wade, N. O. Belser, Conversion of oxyhemoglobin to methemoglobin by organic and inorganic reductants Biochemistry. ,vol. 17, pp. 225- 231 ,(1978) , 10.1021/BI00595A005
Kalyan K. Mukherjea, Md. Selim, Isolation and purification of a vertebral hemoglobin, the chicken hemoglobin, and its interaction with copperglutathione complex Transition Metal Chemistry. ,vol. 30, pp. 496- 498 ,(2005) , 10.1007/S11243-005-1032-3
Michael D. Waters, H.Frank Stack, Marcus A. Jackson, Herman E. Brockman, Silvio De Flora, Activity profiles of antimutagens: in vitro and in vivo data. Mutation Research. ,vol. 350, pp. 109- 129 ,(1996) , 10.1016/0027-5107(95)00097-6
G. Hild, M. Nyitrai, R. Gharavi, B. Somogyi, J. Belágyi, Fluorescence quenching of the tryptophan emission from the F- and G-forms of actin Journal of Photochemistry and Photobiology B-biology. ,vol. 35, pp. 175- 179 ,(1996) , 10.1016/S1011-1344(96)07319-8