Genetic recombination of bacterial plasmid DNA: Physical and genetic analysis of the products of plasmid recombination in Escherichia coli

摘要: Derivatives of plasmid pBR322 DNA containing tet mutations were constructed by inserting XhoI linkers at various sites in the tetracycline resistance gene. Monomer plasmids either tet-10 allele located nucleotide position 23 or tet-14 1267 used to construct a circular dimer one copy each and trimer two copies allele. Genetic recombination these DNAs produce functional gene could be detected as production tetracycline-resistant progeny during growth transformants using restriction mapping assay which rearrangement mutant alleles. The structure individual products was determined mapping. This analysis suggested that many 70% events Escherichia coli AB1157 have involved conversion events. formation most easily predicted model involving figure 8 intermediates symmetric regions heteroduplex. Recombination JC10287 delta(srlR-recA)304 occurred 5% wild-type frequency appeared occur similar mechanism. JC9604 recA56 recB21 recC22 sbcA23 20 times involve multiple independent