Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls.

摘要: Abstract Different morphological types of rumen bacteria which degraded cell walls forage grasses with various in vitro digestibilities were evaluated electron microscopy. The majority these (i.e., about 70% or more) consisted two distinct types: (i) encapsulated cocci and (ii) irregularly shaped bacteria, resembling major fiber digesters found the rumen. Each type was capable degrading structurally intact walls. Differences (P less than equal to 0.02) percent ratio observed between Bermuda grass fescue; orchard similar variations high. proportion usually increased time digestion. greater 0.1) not percentage attached specific tissue either fescue. However, tended be more prevalent on sclerenchyma other tissues grass, but Transmission microscopy digestion rapidly blades revealed that mesophyll, parenchyma bundle sheath, parts epidermal wall apparently without direct attachment although near undergoing Anaerobic growth studies showed total culturable developing medium 10 media containing carbohydrates those pectin, xylan, cellobiose) 80% higher from bacterial populations adapted compared grass; number colonies grass-adapted population significantly 0.05) xylan. Filter paper tests cellulolytic activity fescue grass.