Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing

摘要: Fungal plant pathogens secrete effector molecules to establish disease on their hosts, and plants in turn use immune receptors try intercept these effectors. The tomato receptor Ve1 governs resistance race 1 strains of the soil-borne vascular wilt fungi Verticillium dahliae albo-atrum, but corresponding remained unknown thus far. By high-throughput population genome sequencing, a single 50-Kb sequence stretch was identified that only occurs strains, subsequent transcriptome sequencing Verticillium-infected Nicotiana benthamiana revealed highly expressed ORF this region, designated Ave1 (for Avirulence tomato). Functional analyses confirmed activates Ve1-mediated demonstrated markedly contributes fungal virulence, not also Arabidopsis. Interestingly, is homologous widespread family natriuretic peptides. Besides plants, proteins were found bacterial pathogen Xanthomonas axonopodis pathogenic Colletotrichum higginsianum, Cercospora beticola, Fusarium oxysporum f. sp. lycopersici. distribution homologs, coincident with presence within flexible genomic strongly suggests acquired from through horizontal gene transfer. Remarkably, by transient expression we show homologs F. C. beticola can activate resistance. In line observation, mediate toward tomato, showing involved against multiple pathogens.