T cell receptor γ cDNA in human fetal liver and thymus : variable regions of γ chains are restricted to VγI or V9, due to the absence of splicing of the V10 and V11 leader intron

摘要: Although complete in-frame transcripts of the human T cell receptor gamma V10 and V11 genes have been described, corresponding chains never found in delta receptors. In this study, we show that leader intron all cDNA isolated from fetal thymus, liver adult peripheral blood lymphocytes are unspliced. We demonstrate that, due to absence splicing, pseudogenes cannot be expressed chains. They first type described a rearranging receptor/immunoglobulin locus. Therefore repertoire at protein level is limited subgroup V I V9. By analysis polymerase chain reaction products total cDNA, find locus active early ontogeny (8 weeks), as shown by presence rearranged V9 gene liver. At 13 weeks, well undergone productive rearrangements liver, thymus. Most rearrangements, if not all, involve C1 region (JP1, JP, J1 segments) both tissues, confirming accessibility stages development.