Krüppel-like Factor 7 engineered for transcriptional activation promotes axon regeneration in the adult corticospinal tract
作者： M. G. Blackmore , Z. Wang , J. K. Lerch , D. Motti , Y. P. Zhang
关键词:
摘要: Axon regeneration in the central nervous system normally fails, part because of a developmental decline intrinsic ability CNS projection neurons to extend axons. Members KLF family transcription factors regulate regenerative potential developing neurons. Expression one member, KLF7, is down-regulated developmentally, and overexpression KLF7 cortical vitro promotes axonal growth. To circumvent difficulties achieving high neuronal expression exogenous we created chimera with VP16 transactivation domain, which displayed enhanced compared native protein while maintaining transcriptional activation growth promotion vitro. Overexpression VP16-KLF7 overcame loss slice cultures. Adult corticospinal tract (CST) failed up-regulate response axon injury, vivo promoted both sprouting CST adult mice. These findings identify unique means promoting by reengineering developmentally down-regulated, growth-promoting factor.
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