Long-term sustainable dendritic cell-specific depletion murine model for periodontitis research

摘要: Dendritic cells (DCs) are specialized antigen-presenting that play a pivotal role in the pathogenesis of periodontitis. The use animal models to study DCs periodontitis has been limited by lack method for sustained depletion DCs. Hence, objectives this were validate zDC-DTR knockin mouse model conventional (cDCs) depletion, as well investigate whether could be long enough induce alveolar bone loss model. mice treated with different dose regimens diphtheria toxin (DT) determine survival rate. A loading DT 20ng/bw, followed and maintained doses 10ng/bm every 3days up 4weeks demonstrated 80% survival. Animals weighed weekly peripheral blood was obtained confirm normal neutrophil counts. Five animals per group euthanized at baseline, 24h, 1 4weeks. Bone marrow (BM), spleen (SP) gingival tissue (GT) harvested, isolated, separated stained Pre-DCs precursors (CD45R-MHCII+CD11c+Flt3+CD172a+) BM, cDCs (CD11c+MHCII+CD209+) spleen, GT (CD45R+MHCII+CD11c+ DC-SIGN/CD209+). BM significantly depleted 24h 4weeks, compared blank (PBS) controls. Circulating non-significant trend deplete 1week high variability among mice. also showed similar 24h. seems viable evaluating immune homeostasis disruption response long-term oral infection.