Long-term cross-species brain transplantation of a polymer-encapsulated dopamine-secreting cell line.

摘要: Cross-species transplantation of dopamine-releasing cell lines protected against immune rejection by a semi-permeable synthetic membrane may provide source neurotransmitters for the treatment Parkinson's disease. Experiments were carried out to assess whether polymer-encapsulated PC12 cells, catecholaminergic line derived from rat pheochromocytoma, could survive in vitro as well vivo after implantation striatum adult guinea pigs. When maintained vitro, encapsulated cells exhibited good survival, proliferated, and spontaneously released dopamine at least 6 months. They also retained capacity depolarization-elicited release. In vivo, well-preserved tyrosine hydroxylase-positive observed capsules implanted 4, 8, 12 weeks. Unencapsulated or nonintact did not any these time periods. The survival transplanted across species suggests that polymer encapsulation an alternative xenotransplantation secretory absence systemic immunosuppression.