14-3-3ζ Regulation of Metastasis Through Mediation of Liprin-α and Liprin-β

摘要: 14-3-3ζ Regulation of Metastasis Through Mediation Liprin-α and Liprin-β Rachel Hynes Department Chemistry Biochemistry, BYU Master Science Cancer is a set varied diverse diseases that share common characteristics, such as active proliferation, increased replicative potential, tissue invasion or metastasis. One protein, 14-3-3ζ, shown to be upregulated in number different cancers also correlates with poor patient prognosis, recurrence, mortality. This protein comes from family adapter proteins known for their scaffolding ability, proand anti-oncogenic capabilities, affinity phosphorylated substrates. It has been previously participate cancer progression, subversion apoptosis, increase chemoresistance. Herein we will discuss the ability promote distant-site metastasis propose it does so through variety mechanisms including MAPK signaling cascade, HER2/ErbB2 pathway, by mediation cell adhesion regulation LAR. was identified novel interactor mass spectrometry-based interactomics analysis. found co-immunoprecipitate both Liprin-β. We identification mutation putative binding sites on Liprins, effect these have Liprins LAR, possible downstream consequences interactions. The results described herein are inconclusive due part our inability obtain reliable pulldown identify 14-3-3ζ-binding concluding chapter contains discussion future directions, creation further Liprin mutants well fluorescent imaging LAR localization focal turnover.