Precision of Multiple Reaction Monitoring Mass Spectrometry Analysis of Formalin-Fixed, Paraffin-Embedded Tissue

摘要: We compared the reproducibility of multiple reaction monitoring (MRM) mass spectrometry-based peptide quantitation in tryptic digests from formalin-fixed, paraffin-embedded (FFPE) and frozen clear cell renal carcinoma tissues. The analyses targeted a candidate set 114 peptides previously identified shotgun proteomic analyses, which 104 were detectable FFPE tissue. Although signal intensities for MRM tissue on average 66% those tissue, median coefficients variation (CV) measurements tissues nearly identical (18–20%). Measurements lysine C-terminal arginine similarly reproducible (19.5% 18.3% CV, respectively). further evaluated precision MRM-based by analysis Her2 receptor overexpressing mouse xenograft model breast cancer human sp...