Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology.
作者： Dongsheng Liu , David Cowburn
DOI: 10.1007/978-1-4939-6451-2_9
关键词:
摘要: Segmental isotopic labeling of samples for NMR studies is attractive large complex biomacromolecular systems, especially function-related protein-ligand interactions and protein dynamics (Goto Kay, Curr Opin Struct Biol 10:585-592, 2000; Rosa et al., Molecules (Basel, Switzerland) 18:440, 2013; Hiroaki, Expert Drug Discovery 8:523-536, 2013). Advantages segmental include selective examination specific segment(s) within a by NMR, significantly reducing the spectral complexity proteins, allowing application variety solution-based strategies. By utilizing intein techniques (Wood Camarero, J Chem 289:14512-14519, 2014; Paulus, Annu Rev Biochem 69:447-496, 2000), two related approaches can generally be used in proteins: expressed ligation (Muir, 72:249-289, 2003) trans-splicing (Shah Am Soc 134:11338-11341, 2012). Here, we describe general implementation latest improvements method production labeled samples.
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Shaorong Chong, Kay S. Williams, Chad Wotkowicz, Ming-Qun Xu, Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein Journal of Biological Chemistry. ,vol. 273, pp. 10567- 10577 ,(1998) , 10.1074/JBC.273.17.10567
Brenda Ayers, Ulrich K. Blaschke, Julio A. Camarero, Graham J. Cotton, Mande Holford, Tom W. Muir, Introduction of unnatural amino acids into proteins using expressed protein ligation Biopolymers. ,vol. 51, pp. 343- 354 ,(1999) , 10.1002/(SICI)1097-0282(1999)51:5<343::AID-BIP4>3.0.CO;2-W
Vasant Muralidharan, Kaushik Dutta, Jaehyun Cho, Miquel Vila-Perello, Daniel P. Raleigh, David Cowburn, Tom W. Muir, Solution structure and folding characteristics of the C-terminal SH3 domain of c-Crk-II. Biochemistry. ,vol. 45, pp. 8874- 8884 ,(2006) , 10.1021/BI060590Z
David S. Wishart, Colin G. Bigam, Jian Yao, Frits Abildgaard, H. Jane Dyson, Eric Oldfield, John L. Markley, Brian D. Sykes, 1H, 13C and 15N chemical shift referencing in biomolecular NMR. Journal of Biomolecular NMR. ,vol. 6, pp. 135- 140 ,(1995) , 10.1007/BF00211777
Thomas C. Evans, Jack Benner, Ming-Qun Xu, Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Science. ,vol. 7, pp. 2256- 2264 ,(1998) , 10.1002/PRO.5560071103
Hideo Iwai, Andreas Plückthun, Circular β-lactamase: stability enhancement by cyclizing the backbone FEBS Letters. ,vol. 459, pp. 166- 172 ,(1999) , 10.1016/S0014-5793(99)01220-X
Ming-Qun Xu, Thomas C. Evans, Intein-mediated ligation and cyclization of expressed proteins Methods. ,vol. 24, pp. 257- 277 ,(2001) , 10.1006/METH.2001.1187
Neel H. Shah, Geoffrey P. Dann, Miquel Vila-Perelló, Zhihua Liu, Tom W. Muir, Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering. Journal of the American Chemical Society. ,vol. 134, pp. 11338- 11341 ,(2012) , 10.1021/JA303226X
Derek Macmillan, Lubna Arham, Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation. Journal of the American Chemical Society. ,vol. 126, pp. 9530- 9531 ,(2004) , 10.1021/JA047855M
Tanya Hosfield, Quinn Lu, INFLUENCE OF THE AMINO ACID RESIDUE DOWNSTREAM OF (ASP)4LYS ON ENTEROKINASE CLEAVAGE OF A FUSION PROTEIN Analytical Biochemistry. ,vol. 269, pp. 10- 16 ,(1999) , 10.1006/ABIO.1998.3084