Fluorescence Techniques for the Study of Biological Motion
作者： Dennis E. Koppel
DOI: 10.1007/978-1-4684-4487-2_16
关键词:
摘要: A variety of fluorescence techniques for the study biological motion are described and discussed. We consider three basic strategies: time-resolved emission, photobleaching, correlation spectroscopy. In first these, sample is excited with pulses light short compared to singlet-state lifetime. Molecular motions over time-scale state lifetime characterized through their effects on observed emission. photobleaching technique, intense pulses, under (but very long lifetime), used selectively deplete ground-state population. times by measuring “recovery” after monitored an attenuated CW source. spectroscopy, in contrast, illuminated only monitoring beam. excited-state calculations function spontaneous, stochastic intensity fluctuations about ensemble average.
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