Some Aspects of the Relationship of Peptide Structures to Activity in Pituitary Hormones

摘要: Publisher Summary For any structural studies pursued in connection with hormones or for consideration of protein peptide structure, it must be borne mind that the foundation upon which subsequent rest is isolation particular hormone a highly purified state. Once purity has been established by both physicochemical and biological methods, its structure can then determined; once known, investigator proceed to synthesis. Demonstrating purity, relatively easy task low molecular weight, becomes more complex larger molecules. Furthermore, cannot reiterated too often no single procedure bear full burden proof homogeneity. Not only that, but even agreement among multiplicity criteria gives absolute guarantee, enhances likelihood substance question pure. Those methods applied most success detection microheterogeneity proteins, whose use now current chemists, are ultracentrifugation, electrophoresis, diffusion solubility studies, chromatography, countercurrent distribution, terminal amino acid analysis, immunochemical investigations. It notable some instances, apparently homogeneous every criterion may exist several forms, all possess identical potency, perhaps differ from one another certain other respects, such as amide content, degree denaturation, helical configuration.