Development of immunoaffinity solid phase microextraction probes for analysis of sub ng/mL concentrations of 7-aminoflunitrazepam in urine.

摘要: Abstract We report on the development of solid phase microextraction probes for drug analysis, prepared with antibodies specific benzodiazepines covalently immobilized to surface. In technique, antibody are exposed a sample containing 30 min. Extracted drugs subsequently desorbed from in 500 μL methanolic desorption solution, which is dried, reconstituted small volume injection solution and analysed by LC-MS/MS. The were characterized both before after immobilization, facilitate rational selection such analyses. Polyclonal monoclonal compared as was impact affinity purification polyclonal isolate drug-specific fraction. evaluated utility analyzing 7-aminoflunitrazepam at sub ng/mL concentrations urine, expected be found several days single oral dose 2 mg flunitrazepam. Such analyses required monitoring abuse this drug, terms ‘club drug’ use cases drug-facilitated sexual assault. these blood urine much lower than chronic difficult analyse conventional methods. method developed has limit detection 0.02 ng/mL, accuracy ranging 1% 27% precision (% R.S.D.) 2% 10% between upper limits quantitation analysis urine. dynamic range limited instrument sensitivity, 0.5 ng/mL, approaching capacity probes. This would allow quantitative samples below that measurable many other methods general highly selective screen higher concentrations. similar most sensitive literature specifically designed but advantage significantly simplified preparation. simplification makes technique more amenable professionals non-professionals.