Exposure of Ancistrus multispinis (Regan, 1912, Pisces, Teleostei) to deltamethrin: Effects on cellular immunity

摘要: Abstract The objective of this study performed in Ancistrus multispinis was to evaluate whether sublethal doses deltamethrin (DM) could affect lipopolysaccharide (LPS)-induced leukocyte migration the peritoneal cavity and production nitric oxide (NO) by kidney macrophages exposed LPS and/or DM. For cell studies, 10 fish were DM 0.1 or 0.3 mg kg−1 vehicle for 96 h. After period, each group received (0.1 μg kg−1) saline cells collected after 4 h. NO, isolated from eight incubated 24 48 h with (10 μg mL−1) (0.3 1 μg mL−1). results analyzed using ANOVA followed Bonferroni multiple comparisons test. A small population (530 ± 30.8 cells μL−1) naturally observed cavity, however inoculation LPS, number leukocytes increased 157%. treated 0.3 mg kg−1, total had 100 162%, respectively. combination higher dose showed a potentiating additive effect. NO all groups relation control group. 1 μg mL−1 also other groups. “in vitro” method can be used potential effects toxic agents on immune system. Therefore, we conclude that increase A. multispinis.