FORMATION OF BONE TISSUE IN CULTURE FROM ISOLATED BONE CELLS

摘要: A system is described for the formation of bone tissue in culture from isolated rat cells. The cells were obtained embryonic calvarium and periosteum or traumatized, lifted young rats. cultured a period up to 8 wk, during which time morphological, biochemical, functional properties cultures studied. Formation by these was shown, that demonstrated osteoblastic morphology light electron microscopy, collagen formed similar collagen, there mineralization specific bone, reacted hormone calcitonin increased calcium ion uptake. Calcification fine structure matrix described. Three stages calcification process observed microscopy. It concluded growing vitro are able function way such vivo. This starting therefore suitable studies on bone.