作者: Cecilie Morland , Kaja Nordengen , Max Larsson , Laura M. Prolo , Zoya Farzampour
DOI: 10.1096/FJ.12-206300
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摘要: The mechanism of release and the role l-aspartate as a central neurotransmitter are controversial. A vesicular for has been difficult to prove, no transporter was identified until it found that sialin could transport l-glutamate when reconstituted into liposomes. We sought clarify in this process by combining uptake studies isolated synaptic vesicles with immunocyotchemical investigations hippocampal slices. radiolabeled taken up vesicles. uptake, relative twice high hippocampus whole brain, striatum, entorhinal frontal cortices not inhibited l-glutamate. further show is essential exocytosis l-aspartate, there difference ATP-dependent from sialin-knockout wild-type mice. In addition, expression PC12 cells did result significant vesicle depolarization-induced depletion nerve terminals similar slices Further, evidence nonvesicular via volume-regulated anion channels or plasma membrane excitatory amino acid transporters. This suggests exocytotically released after accumulation other than sialin.—Morland, C., Nordengen, K., Larsson, M., Prolo, L. Farzampour, Z., Reimer, R. J., Gundersen, V. Vesicular independent sialin.