Vesicular uptake and exocytosis of l-aspartate is independent of sialin

作者: Cecilie Morland , Kaja Nordengen , Max Larsson , Laura M. Prolo , Zoya Farzampour

DOI: 10.1096/FJ.12-206300

关键词:

摘要: The mechanism of release and the role l-aspartate as a central neurotransmitter are controversial. A vesicular for has been difficult to prove, no transporter was identified until it found that sialin could transport l-glutamate when reconstituted into liposomes. We sought clarify in this process by combining uptake studies isolated synaptic vesicles with immunocyotchemical investigations hippocampal slices. radiolabeled taken up vesicles. uptake, relative twice high hippocampus whole brain, striatum, entorhinal frontal cortices not inhibited l-glutamate. further show is essential exocytosis l-aspartate, there difference ATP-dependent from sialin-knockout wild-type mice. In addition, expression PC12 cells did result significant vesicle depolarization-induced depletion nerve terminals similar slices Further, evidence nonvesicular via volume-regulated anion channels or plasma membrane excitatory amino acid transporters. This suggests exocytotically released after accumulation other than sialin.—Morland, C., Nordengen, K., Larsson, M., Prolo, L. Farzampour, Z., Reimer, R. J., Gundersen, V. Vesicular independent sialin.

参考文章(81)
David J. Rossi, Takeo Oshima, David Attwell, Glutamate release in severe brain ischaemia is mainly by reversed uptake Nature. ,vol. 403, pp. 316- 321 ,(2000) , 10.1038/35002090
S Naito, T Ueda, Adenosine triphosphate-dependent uptake of glutamate into protein I-associated synaptic vesicles. Journal of Biological Chemistry. ,vol. 258, pp. 696- 699 ,(1983) , 10.1016/S0021-9258(18)33100-4
Tatiana Brustovetsky, Matthew K. Brittain, Patrick L. Sheets, Theodore R. Cummins, Vsevolod Pinelis, Nickolay Brustovetsky, KB-R7943, an inhibitor of the reverse Na+ /Ca2+ exchanger, blocks N-methyl-D-aspartate receptor and inhibits mitochondrial complex I. British Journal of Pharmacology. ,vol. 162, pp. 255- 270 ,(2011) , 10.1111/J.1476-5381.2010.01054.X
Vidar Gundersen, Farrukh A. Chaudhry, Jan G. Bjaalie, Frode Fonnum, Ole Petter Ottersen, Jon Storm-Mathisen, Synaptic vesicular localization and exocytosis of L-aspartate in excitatory nerve terminals: a quantitative immunogold analysis in rat hippocampus. The Journal of Neuroscience. ,vol. 18, pp. 6059- 6070 ,(1998) , 10.1523/JNEUROSCI.18-16-06059.1998
Frans W. Verheijen, Elly Verbeek, Nina Aula, Cecile E.M.T. Beerens, Adrie C. Havelaar, Marijke Joosse, Leena Peltonen, Pertti Aula, Hans Galjaard, Peter J. van der Spek, Grazia M.S. Mancini, A new gene, encoding an anion transporter, is mutated in sialic acid storage diseases Nature Genetics. ,vol. 23, pp. 462- 465 ,(1999) , 10.1038/70585
Kaja Nordengen, Vidar Gundersen, Cecilie Morland, Aleksander Talgøy Holten, Vesicular Release of L- and D-Aspartate from Hippocampal Nerve Terminals: Immunogold Evidence The Open Neuroscience Journal. ,vol. 2, ,(2008)
H.T. McMahon, P Foran, J.O. Dolly, M Verhage, V.M. Wiegant, D.G. Nicholls, Tetanus toxin and botulinum toxins type A and B inhibit glutamate, gamma-aminobutyric acid, aspartate, and met-enkephalin release from synaptosomes. Clues to the locus of action. Journal of Biological Chemistry. ,vol. 267, pp. 21338- 21343 ,(1992) , 10.1016/S0021-9258(19)36614-1
John W. Phillis, Michael H. O’Regan, Hyperglycemia and Extracellular Glutamate in the Ischemic Brain Stroke. ,vol. 31, pp. 1457- 1466 ,(2000) , 10.1161/01.STR.31.6.1457-C
P R Maycox, T Deckwerth, J W Hell, R Jahn, Glutamate uptake by brain synaptic vesicles. Energy dependence of transport and functional reconstitution in proteoliposomes. Journal of Biological Chemistry. ,vol. 263, pp. 15423- 15428 ,(1988) , 10.1016/S0021-9258(19)37605-7
S Zappettini, M Grilli, A Salamone, E Fedele, M Marchi, Pre-synaptic nicotinic receptors evoke endogenous glutamate and aspartate release from hippocampal synaptosomes by way of distinct coupling mechanisms British Journal of Pharmacology. ,vol. 161, pp. 1161- 1171 ,(2010) , 10.1111/J.1476-5381.2010.00958.X