Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2.

摘要: Escherichia coli CRT4624-P2sig5 is a dnaA mutant in which integration of the prophage P2sig5 has occurred at attP2II site (min 85). This strain was integratively suppressed, and when cells were shifted to 42 degrees C replication initiated or near P2 prophage. Initially, this primarily direction that corresponds clockwise on genetic map. Replication also counterclockwise direction, but initiation approximately 40 min later than other direction. Because delay, forks traveled first arrive region terminus. These ceased aroD locus 37), it proposed terminus between rac loci 31). A model for cycle chromosome C.