Silencing of PTK7 in Colon Cancer Cells: Caspase-10-Dependent Apoptosis via Mitochondrial Pathway
作者： Ling Meng , Kwame Sefah , Meghan B. O'Donoghue , Guizhi Zhu , Dihua Shangguan
DOI: 10.1371/JOURNAL.PONE.0014018
关键词:
摘要: Protein tyrosine kinase-7 (PTK7) is a catalytically inactive receptor kinase (RTK). PTK7 upregulated in many common human cancers, including colon cancer, lung gastric cancer and acute myeloid leukemia. The reason for this up-regulation not yet known. To explore the functional role of PTK7, expression HCT 116 cells was examined using small interference (siRNA)-mediated gene silencing. Following transfection, siRNA successfully suppressed mRNA protein expression. Knocking down inhibited cell proliferation compared to control groups induced apoptosis. Furthermore, apoptosis characterized by decreased mitochondrial membrane potential activation caspase-9 -10. Addition caspase-10 inhibitor totally blocked apoptosis, suggesting that may play critical PTK7-knockdown-induced downstream mitochondria. These observations indicate provide therapeutic pathway treatment variety cancers.
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