Microglia activation in the extremely preterm human brain

摘要: BACKGROUND The periventricular white matter (PVWM) of the immature preterm brain is selectively vulnerable to a spectrum injury. Although essential for normal development, presence resident microglia may exacerbate PVWM METHODS We used immunohistochemistry investigate profile in human noninjured control brains and with evidence germinal matrix hemorrhage/intraventricular hemorrhage (GMH/IVH), median gestational age (GA) 24.1 25.4 wk, respectively. RESULTS number was higher than other regions both GMH/IVH groups. Microglial density increased further brains, regardless severity despite macroscopic imaging appearances PVWM. This due an increase activated Iba1/CD68- not Iba/CD45-immunopositive microglia. However, there were very few CD68/Ki67 colocalized cells, suggesting that source this be quick transformation CD45-immunopositive hematopoietic into CD68-immunopositive There also apoptosis all cases GMH/IVH, axonal injury tumor necrosis factor-α (TNF-α) expression evident most severe cases. CONCLUSION Isolated influence ongoing significant role played by microglial activation.