Molecular characterization of a plasmid-borne (pTC82) chloramphenicol resistance determinant (cat-TC) from Lactobacillus reuteri G4.

摘要: Lactobacillus reuteri G4 contains a 7.0-kb plasmid (pTC82) encoding resistance to chloramphenicol (Cm). Determination of the nucleotide sequence genetic determinant (cat-TC) Cm on pTC82 revealed an open reading frame for 238-amino-acid acetyltransferase (CAT) monomer. This structural cat gene, 714 bp in length, was highly related (ca. 95% and ca. 81% amino acid identity) 648-bp gene from Staphylococcus aureus pC194. A total 6 transversions 4 deletions observed along whole DNA cat-TC compared that cat-pC194. To determine activity putative recombinant pUC8217 containing subjected maxicell analysis. The molecular mass synthesized protein, based electrophoretic mobility, reasonable agreement with 27.3 kDa predicted sequence. is first reported Cm-resistance L. also evidence adding list versatile bacterial genera which naturally acquire cat-pC194 microbial ecological system.