Human mast cell tryptase activates single-chain urinary-type plasminogen activator (pro-urokinase)
作者： M.S. Stack , D.A. Johnson
DOI: 10.1016/S0021-9258(17)36896-5
关键词:
摘要: Human lung mast cell tryptase is a trypsin-like serine proteinase that stored in granules and released by activated cells. Here we report potent activator of single-chain urinary-type plasminogen (scu-PA, or prourokinase), the zymogen form (u-PA). Activation was complete within 75 min using an enzyme:substrate molar ratio 1:50 accompanied cleavage scu-PA at Lys158-Ile159, generating active two-chain u-PA. The reaction dependent on enzyme concentration obeyed Michaelis-Menten kinetics. Kinetic constants calculated for activation are Km = 34 microM, Vmax 3.6 pmol u-PA/min, kcat 0.08 s-1. These data suggest from tumor-associated cells may participate scu-PA.
sci-hub.st 参考文章(33)
K. Danø, P.A. Andreasen, J. Grøndahl-Hansen, P. Kristensen, L.S. Nielsen, L. Skriver, Plasminogen activators, tissue degradation, and cancer. Advances in Cancer Research. ,vol. 44, pp. 139- 266 ,(1985) , 10.1016/S0065-230X(08)60028-7
V Gurewich, R Pannell, Inactivation of single-chain urokinase (pro-urokinase) by thrombin and thrombin-like enzymes: relevance of the findings to the interpretation of fibrin-binding experiments Blood. ,vol. 69, pp. 769- 772 ,(1987) , 10.1182/BLOOD.V69.3.769.769
T Suyama, K Fujikawa, A Ichinose, The activation of pro-urokinase by plasma kallikrein and its inactivation by thrombin. Journal of Biological Chemistry. ,vol. 261, pp. 3486- 3489 ,(1986) , 10.1016/S0021-9258(17)35674-0
K F Austen, D Seldin, R A Lewis, L B Schwartz, Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. Journal of Immunology. ,vol. 126, pp. 1290- 1294 ,(1981)
T C Wun, L Ossowski, E Reich, A proenzyme form of human urokinase. Journal of Biological Chemistry. ,vol. 257, pp. 7262- 7268 ,(1982) , 10.1016/S0021-9258(18)34566-6
T J Smith, M W Hougland, D A Johnson, Human lung tryptase. Purification and characterization. Journal of Biological Chemistry. ,vol. 259, pp. 11046- 11051 ,(1984) , 10.1016/S0021-9258(18)90620-4
Chris Nolan, Lucinda S. Hall, Grant H. Barlow, Ilse I.E. Tribby, Plasminogen activator from human embryonic kidney cell cultures. Evidence for a proactivator. Biochimica et Biophysica Acta. ,vol. 496, pp. 384- 400 ,(1977) , 10.1016/0304-4165(77)90321-X
D C Rijken, M Hoylaerts, D Collen, Fibrinolytic properties of one-chain and two-chain human extrinsic (tissue-type) plasminogen activator. Journal of Biological Chemistry. ,vol. 257, pp. 2920- 2925 ,(1982) , 10.1016/S0021-9258(19)81052-9
V Ellis, M F Scully, V V Kakkar, Plasminogen Activation Initiated by Single-chain Urokinase-type Plasminogen Activator Journal of Biological Chemistry. ,vol. 264, pp. 2185- 2188 ,(1989) , 10.1016/S0021-9258(18)94159-1
H R Lijnen, B Van Hoef, L Nelles, D Collen, Plasminogen activation with single-chain urokinase-type plasminogen activator (scu-PA). Studies with active site mutagenized plasminogen (Ser740----Ala) and plasmin-resistant scu-PA (Lys158----Glu). Journal of Biological Chemistry. ,vol. 265, pp. 5232- 5236 ,(1990) , 10.1016/S0021-9258(19)34111-0