Occurrence and characterization of quinolone resistant Escherichia coli from Norwegian turkey meat and complete sequence of an IncX1 plasmid encoding qnrS1.

摘要: Plasmid-mediated quinolone resistance (PMQR) is frequent among Escherichia coli from various food products and animals in several countries. The objective of this study was to characterize resistant E. (QREC) Norwegian turkey meat regarding profiles, genetic mechanisms for resistance, relatedness, investigate whether PMQR genes were present. In total, 78 QREC isolated by a selective method 156 samples throughout 2013. Isolates subjected susceptibility testing, characterization mechanisms, serotyping, phylotyping multi-locus variable-tandem repeat analysis (MLVA). All isolates ciprofloxacin, while 77 also nalidixic acid. acid sensitive isolate had profile indicating the presence gene. Both PCR whole genome sequencing confirmed 47 304 kb IncX1 plasmid containing qnrS1 mechanism conferring remaining mediated mutations resistance-determining region chromosomal gyrA gene most parC Molecular typing MLVA showed high degree diversity, although four clusters dominated. Two contained strains belonging phylogroup D/serogroup O176, third B1/serogroup O19, whereas fourth B1/non-typeable serogroup. within latter cluster profiles identical day-old imported parent investigated preliminary at Veterinary Institute. This finding suggests that obtained may have been introduced via import breeding Norway. first time described meat. Compared available carrying plasmids Genbank, current similarity other both Shigella flexneri found different geographical areas sources. To conclude, are main meat, has not widely dispersed population turkey.