Bioactive nanocomposite coatings under visible light illumination promoted surface-mediated gene delivery.
作者： Lili Yao , Xiaozhao Wang , Wenjian Weng , Yongqing Fu , Kui Cheng
DOI: 10.1039/D0BM00123F
关键词:
摘要: Gene delivery based on bioactive coatings collagen has great potential for applications in bone repair. Meanwhile, controlled gene at specific times/regions is essential an efficient and complete reconstruction process. However, spatio-temporal regulation of release remains a challenge. In this paper, we used visible light illumination to effectively regulate subsequent into biological cells. A responsive nanocomposite coating (based collagen/gold nanoparticles, e.g., Col/AuNPs) was prepared through hydrothermal sol–gel processes as loading platform complexes enhanced green fluorescent protein Lipofectamine2000 (LF/GFP). The results showed that the amount immobilized LF/GFP increased Col/AuNPs pre-adsorbed significantly manner under illumination. Moreover, cellular intake released genes improved, thus enhancing expression efficiency mechanism attributed changes structures rearrangement cytoskeletal induced by photothermal effect. developed Col/AuNP composite effective both surface-mediated gene-mediated
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