Activation of transcription by guanosine 5'-diphosphate,3'-diphosphate, transfer ribonucleic acid, and novel protein from Escherichia coli.

摘要: A protein factor TFms) that is required for ppGpp to stimulate RNA synthesis has been purified from an eluate of crude ribosomes. TFms also the capacity without present. Under standard conditions action and requires uncharged tRNA. act at inhibition promote formation rifampicin-resistant or polytrI)-resistant preinitiation complexes. In presence rifampicin poly(rI), tRNA no longer required. With lambdah80dlacPs DNA as template, together with stimulated gal a much greater extent than total synthesis. The stimulation both lac gel was increased in cyclic AMP receptor AMP.