Common bean (Phaseolus vulgaris L.) hydrolysates inhibit inflammation in LPS-induced macrophages through suppression of NF-κB pathways

摘要: Abstract The objectives of this study were to evaluate the antioxidant capacity protein hydrolysates common bean (Phaseolus vulgaris L.) varieties Negro 8025 and Pinto Durango determine their effect on markers inflammation in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Cell viability was determined percentage viable cells calculated concentrations that allowed >80% cell used inflammation. Alcalase pepsin–pancreatin showed highest after 80 120 min hydrolysis, respectively. at inhibited inflammation, with IC50 values 34.9 ± 0.3, 13.9 ± 0.3, 5.0 ± 0.1 3.7 ± 0.2 μM, while var. needed 43.6 ± 0.2, 61.3 ± 0.3, 14.2 ± 0.3 48.2 ± 0.1 μM for inhibition cyclooxygenase-2 expression, prostaglandin E2 production, inducible nitric oxide synthase expression Also, significantly transactivation NF-κB nuclear translocation p65 subunit. In conclusion, from can be combat inflammatory oxidative-associated diseases.