Reduced number of neurons in the hippocampus and the cerebellum in the postnatal guinea-pig following intrauterine growth-restriction.

摘要: Intrauterine growth restriction is a risk factor for neurological and behavioural deficits in children although the precise underlying biological correlate this unclear. The present study shows that animals with intrauterine restriction, induced by period of reduced placental blood flow during second half pregnancy, demonstrate numbers neurons hippocampus cerebellum conjunction retarded dendritic axonal within these structures. Intrauterine was at 30 days gestational age unilateral uterine artery ligation pregnant guinea-pigs. At one week age, total number CA1 pyramidal Purkinje were determined using combined fractionator/optical disector technique. Cavalieri Principle used to determine volume specific regions cerebellum. body weight classified as growth-restricted 42% (n=8) compared control (n=8, P<0.001), while there smaller effect on brain (16% reduction, P<0.01). Estimates showed reduction (4.19±0.43×105) (5.20±0.44×105, P<0.01), stratum oriens layer above region, which contains apical dendrites neurons, 21% (P<0.01) animals. In (3.97±0.50×105) (5.13±0.52×105, molecular (17%, P<0.05), internal granular (22%, P<0.01) cerebellar white matter (23%, P<0.01). These results show insufficiency pregnancy can development way could lead postnatal animal.