Microtiter plate tests for segregation of bioluminescent bacteria

摘要: It has been recently shown that bioluminescence imaging can be usefully applied to provide new insights into bacterial self-organization. In this work we employ record images of nutrient rich liquid cultures the lux-gene reporter Escherichia coli in microtiter plate wells. The show patterns inhomogenous form along three-phase contact lines. paper analyzes dependencies average number luminous aggregates (clouds) on various environmental factors. particular, our results optimal (neutral) pH and high aeration rates determine highest mean clouds, spatiotemporal do not buffered suspensions. addition, a sigmoidal (switch-like) dependence rate was observed. obtained data interpreted by employing Keller-Segel-Fisher (KSF) model chemotaxis logistic growth, adapted systems metabolically flexible (two-state) bacteria. modified KSF successfully simulated observed switch-like responses. tests their simulations indicate segregation bacteria with different activities proceeds line region.