Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate

摘要: The invention discloses a screening method of resisting joint toxicity heavy metals by trisodium citrate dehydrate. comprises the following steps: step one, preparing mixed solution multiple metal pollutants; two, determining LC50 MTT; three, taking dehydrate as an antagonist, sequentially mixing water solutions with different concentrations to be screened and concentration capable causing death half number cells, adding mixture into culture medium used cell line cultured; four, testing survival activity MTT namely, poisoning for 24h, MTT, incubating 3-4h, measuring thus obtaining efficiency chemical antagonists screened. has advantages that rapid is high, cost low, can simultaneously, so high.